Neutrons paint atomic portrait of prototypical cell signaling enzyme—protein kinase A
Direct observations of the structure and catalytic mechanism of a prototypical kinase enzyme—protein kinase A or PKA—will provide researchers and drug developers with significantly enhanced abilities to understand and treat fatal diseases and neurological disorders such as cancer, diabetes, and cystic fibrosis.
The discovery was made by an international team of researchers using macromolecular neutron crystallography at the Department of Energy’s Oak Ridge National Laboratory (ORNL) and the Institut Laue-Langevin (ILL) in Grenoble, France. Building on a decade-long investigation, the combined efforts revealed previously unknown characteristics of the archetypal protein kinase, including a complete map of the atomic structure and the underpinnings of the enzyme’s chemistry used in cellular signaling. The details are published in the journal Science Advances.
Kinases are a large group of hundreds of enzymes responsible for initiating and regulating a myriad of cellular processes. Kinases send signals to proteins by way of phosphorylation—essentially adding a reactive chemical group consisting of phosphorus and oxygen to a specific location on a substrate protein to activate its specific biological function.
Problems arise when gene mutations occur that cause kinases to work improperly, which in turn leads to disease. For example, a malfunctioning kinase, or one that can’t be deactivated, could be responsible for the overexpression of proteins that lead to the uncontrollable propagation of cancer cells. The opposite is also true in neurological disorders in which a malfunctioning kinase simply causes cells to die.
“Creating drugs that target specific kinases is extremely important,” said ORNL co-author Andrey Kovalevsky. “This detailed understanding of the PKA structure and its dynamics will tell us more about other kinds of kinases and should help drug developers design new drugs with better specificity, which would ultimately translate to more precise therapeutics with fewer side effects.”
Changes in the protein structure due to mutations can be difficult to detect using x-ray scattering, even though the effects of mutations on the protein dynamical behavior can be quite profound. However, these dynamical behaviors become more apparent by examining the overall hydrogen bonding network throughout the protein.
Neutrons are uniquely sensitive to light elements such as hydrogen. Because approximately 50% of all atoms in proteins are hydrogen, and most of the chemical reactions that enzymes catalyse involve hydrogen, neutrons are an ideal and unsurpassed probe for detecting the positions of hydrogen atoms within the protein structure and tracking their movement during catalysis.
Preliminary neutron diffraction tests were made using the diffractometer at ILL. These data enabled researchers to see the exact positions of hydrogen atoms to reveal the complete kinase structure as well as the protonation states of all amino acids in PKA and the ligands bound to its active site, many of which were previously unknown.diffractometer at ORNL’s High Flux Isotope Reactor with full data collection performed using the
Matthew Blakeley, LADI-III beamline scientist at the ILL and co-author of the study, said “Given the relatively large unit-cell of PKA and the fairly small crystal volumes available (0.1mm3), neutron diffraction data collection to sufficiently high-resolution to reveal the protonation states of PKA was only possible using the LADI-III beamline at ILL, and therefore emphasises the importance of international scientific collaborations.”
“Neutrons enabled us to validate x-ray predictions of hydrogen atom positions, as well as determining the protonation states of chemical groups that were not known, or in places where we didn’t expect to find them. This major chemical advance paves the way for more sophisticated molecular modeling and simulation studies of protein kinase structure and function.” said University of California–San Diego corresponding author Susan Taylor.
“Neutrons offer an unprecedented view of the hydrogen bond network surrounding and within the active site of PKA and give crucial insights on how allosteric transmission of information through the protein structure occurs,” added University of Minnesota co-author Gianluigi Veglia. “Combining neutron crystallography with nuclear magnetic resonance spectroscopy will provide a comprehensive understanding of how enzymes work.”
Re. Gerlits et al., Sci. Adv., 2019; 5: eaav0482. DOI: 10.1126/sciadv.aav0482
Contact: Dr M. Blakeley, ILL
ILL Instrument : LADI-III, the quasi-Laue diffractometer
Notes to Editors:
- The paper’s co-authors include lead author Oksana Gerlits, Bredesen Center of the University of Tennessee–Knoxville; Kevin L. Weiss and Andrey Kovalevsky, ORNL; Matthew P. Blakeley, Institut Laue Langevin, Grenoble, France; Gianluigi Veglia, University of Minnesota; and corresponding author Susan S. Taylor, University of California–San Diego, who initiated this effort through a joint project funded by the University of California and Los Alamos National Laboratory.
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- LADI-III is a quasi-Laue neutron diffractometer used for single-crystal studies of biological macromolecules at high resolution (1.3 – 2.5Å) in order to locate individual hydrogen or deuterium atoms of special interest, water structures or other small molecules that can be marked with deuterium to be particularly visible. Data collection is feasible for samples with unit-cell edges ranging from 50 to 150Å using crystal volumes from ~0.05 to 0.5mm3, respectively. You can find out more here: .
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About ILL – the Institut Laue-Langevin (ILL) is an international research centre based in Grenoble, France. It has led the world in neutron-scattering science and technology for almost 40 years, since experiments began in 1972. ILL operates one of the most intense neutron sources in the world, feeding beams of neutrons to a suite of 40 high-performance instruments that are constantly upgraded. Each year 1,200 researchers from over 40 countries visit ILL to conduct research into condensed matter physics, (green) chemistry, biology, nuclear physics, and materials science. The UK, along with France and Germany is an associate and major funder of the ILL.
About ORNL – Oak Ridge National Laboratory (ORNL) operates two neutron source facilities, the High Flux Isotope Reactor and the Spallation Neutron Source. Built and funded by the U.S. Department of Energy (DOE) Office of Basic Energy Sciences (BES), the two facilities combined house 30 neutron scattering instruments, providing researchers with unmatched capabilities for understanding the structure and properties of materials, macromolecular and biological systems, and the fundamental physics of the neutron. More than 1,200 unique users from around the world use ORNL’s neutron sources annually. ORNL is managed and operated by UT-Battelle for DOE. For more information visit