Protocols

General

• Preparation of specifically labelled Purple Membranes

• Phospholipid deuteration protocols for NMR and neutron studies (Oxford)

• Na-9-fluorenylmethoxyloxcarbonyl amino acids – general procedure (Oxford)

• Perdeuteration of the mechanosensitive channel protein MscL (Oxford/ILL)

• Protocol for production of perdeuterated chromosomal DNA (ILL/Keele/EMBL Grenoble)

• Protocol for the synthesis of a selectively-labelled analogue of the alternating copolymer poly[d(A-T)].poly[dA-T)] (ILL/Keele/EMBL Grenoble)

• Protocol for the production of selectively trp-labelled γcrystallin (ILL/EMBL Grenoble)

Scattering length Densities

• Calculation of the Scattering length densities in hydrogenated or/and deuterated conditions (many thanks to Roland May for preparing the spreadsheet)
• calculation of SLD of organic molecules (online server link)

SANS MW

• Calculation of the MW of H-proteins (from Zaccai et al.'s Izero = 0.06 for a Hydrogeated protein of 100KDa at 1 mg/ml, easy excell table from Maxime)

Mass Spectrometry

• Calculation of the percentage of Deuteration (many thanks to JB Artero for preparing the spreadsheet)

NMR Labelling

• ¹⁵N-Labelling of proteins (EMBL Heidelberg)
• ¹³C,¹⁵N-Labelling of proteins
• ²H, ¹³C, ¹⁵N - triple labelling of proteins
• Labelling protocols (CEA Grenoble)

• Spectroscopic protocols: 1 & 2

• Structure protocols 1 & 2

Fermentation protocols (ILL/EMBL)

• Stainless Steel Discolouration and Cleaning - treatment of rusty inox parts of the fermenters

Protocols for growth media (ILL/EMBL)

• Minimal medium
• Rich medium
• Antifoam agents

Protocol for adaptation to D₂O (ILL/EMBL)

• Roche Lab FAQs
• Novagen
• Protocol Online
• Kanamycin insertion kit
• Bowtell Lab manual
• CMS Molecular Biology Resource
• QIAGEN Bench Guide
• Regeneration of commercial nucleic acid extraction columns without the risk of carryover contamination

Molecular biology links/fora

• Molecular Biology Genetics
• Proteomics
• NWFSC Molecular Biology Techniques